Recombinant BRD3 antibody (AA 676-726)

Catalog No. ABIN7455203

This anti-BRD3 antibody is a Rabbit Monoclonal antibody detecting BRD3 in WB, IP, IF (p), ICC, FACS and IHC (fp). Suitable for Human.

Quick Overview for Recombinant BRD3 antibody (AA 676-726) (ABIN7455203)

Target: BRD3 (Bromodomain Containing 3 (BRD3))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BRD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: BLR069G

Product Details anti-BRD3 Antibody

Binding Specificity: AA 676-726

Purpose: Rabbit anti-BRD3 Recombinant Monoclonal Antibody [BLR069G]

Immunogen: between AA 676 and 726

Isotype: IgG

Application Details

Application Notes:

IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 20 μL/1 mg lysate

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 17:40:00

Restrictions: For Research Use only

Handling

Concentration: 100 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for BRD3

Target: BRD3 (Bromodomain Containing 3 (BRD3))

Alternative Name: BRD3

Background: Background: BRD3 (bromodomain containing 3) is a double bromo-domain containing protein. It has been shown to associate with hyperacetylated chromatin of transcribed genes. From this finding it is suggested that BRD3 plays a role in RNA polymerase II transcription through nucleosomes. A chromosomal translocation resulting in a fusion protein involving BRD3 and NUT has been associated with a rare and lethal carcinoma. [taken from the Universal Protein Resource (UniProt) Q15059 and from NCBI Entrez Gene (Gene ID: 8019)].

Gene ID: 8019

NCBI Accession: NP_031397

UniProt: Q15059

Pathways: Chromatin Binding