Recombinant beta Catenin antibody (AA 725-775)

Catalog No. ABIN7455205

The Rabbit Monoclonal anti-beta Catenin antibody has been validated for WB, FACS, IF (p), IP, ICC and IHC (fp). It is suitable to detect beta Catenin in samples from Human and Mouse.

Quick Overview for Recombinant beta Catenin antibody (AA 725-775) (ABIN7455205)

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This beta Catenin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: BLR086G

Product Details anti-beta Catenin Antibody

Binding Specificity: AA 725-775

Purpose: Rabbit anti-beta Catenin Recombinant Monoclonal Antibody [BLR086G]

Immunogen: between AA 725 and 775

Isotype: IgG

Application Details

Application Notes:

IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 20 μL/1 mg lysate

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 17:40:00

Restrictions: For Research Use only

Handling

Concentration: 250 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for beta Catenin

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Alternative Name: beta Catenin

Background: Background: beta Catenin plays an integral role in the Wnt signaling pathway and is critical to morphogenic signaling and body axis specification. Beta Catenin exists in complexes with cadherins, transmembrane proteins that mediate cell adhesion. When Wnt-signaling proteins bind their receptors, beta Catenin is stabilized and accumulates in the cell. Translocation of stabilized beta Catenin allows its association with transcription factors and the activation of specific gene expression.

Gene ID: 1499

NCBI Accession: NP_001895

UniProt: P35222

Pathways: Peptide Hormone Metabolism