CD3EAP antibody (AA 404-454)

Catalog No. ABIN7455215

The Rabbit Polyclonal anti-CD3EAP antibody (ABIN7455215) specifically detects CD3EAP in WB, IP, IF/ICC and IHC (fp). The antibody is reactive with Human samples.

Quick Overview for CD3EAP antibody (AA 404-454) (ABIN7455215)

Target: CD3EAP (CD3e Molecule, epsilon Associated Protein (CD3EAP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD3EAP antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-CD3EAP Antibody

Binding Specificity: AA 404-454

Purpose: Rabbit anti-PAF49 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 404 and 454

Isotype: IgG

Application Details

Application Notes:

ICC-IF: 1:50 - 1:250. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

IHC: 1:500 - 1:2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CD3EAP

Target: CD3EAP (CD3e Molecule, epsilon Associated Protein (CD3EAP))

Alternative Name: PAF49

Background: Background: PAF49 is subunit of RNA polymerase I. PAF49/CAST has been shown to interact with activator upstream binding factor (UBF) and plays an important role in rDNA transcription.

Gene ID: 10849

NCBI Accession: NP_036231

UniProt: O15446