ABL1 antibody (AA 850-900)

Catalog No. ABIN7455217

The Rabbit Polyclonal anti-ABL1 antibody is suitable to detect ABL1 in samples from Human. It has been validated for WB, IP, IF/ICC and IHC (fp).

Quick Overview for ABL1 antibody (AA 850-900) (ABIN7455217)

Target: ABL1 (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-ABL1 Antibody

Binding Specificity: AA 850-900

Purpose: Rabbit anti-cAbl Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 850 and 900

Isotype: IgG

Application Details

Application Notes:

ICC-IF: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. For cytospin preparations of formaldehyde fixed cells permeabilization with Triton-X 100 is recommended.

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: Not recommended. Use rabbit anti-cAbl antibody A301-660A.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for ABL1

Target: ABL1 (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1))

Alternative Name: cAbl

Background: Background: c-Abl is the human cellular homolog of a the v-Abl viral oncogene. v-Abl was originally discovered as a mouse cell-derived sequence in the genome of the Abelson murine leukemia virus (A-MuLV), a transforming retrovirus isolated by the laboratory of Dr. H.T. Abelson. c-Abl is a tyrosine kinase from the Src-family of tyrosine kinases that localizes to both the cytoplasm and nucleus. It bears an SH2 (src-homology 2) and SH3 (src-homology 3) domain responsible for mediating c-Abl protein-protein interactions. c-Abl is implicated to play a role in multiple cellular processes such as cell cycle checkpoint signaling, apoptosis, cell differentiation, cell adhesion, and transcriptional regulation. Chromosomal translocations involving the c-Abl gene are associated with human leukemias.

Gene ID: 25

NCBI Accession: NP_005148

UniProt: P00519

Pathways: Apoptosis, Regulation of Muscle Cell Differentiation, Platelet-derived growth Factor Receptor Signaling, Lipid Metabolism