Nucleolin antibody (C-Term)

Catalog No. ABIN7455226

The Rabbit Polyclonal anti-Nucleolin antibody has been validated for WB, IP, IF (p) and IHC (fp). It is suitable to detect Nucleolin in samples from Human and Mouse.

Quick Overview for Nucleolin antibody (C-Term) (ABIN7455226)

Target: Nucleolin (NCL)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-Nucleolin Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-Nucleolin (NCL) Antibody, Affinity Purified

Predicted Reactivity: Orangutan

Purification: Affinity Purified

Immunogen: between AA 550 and C-term

Isotype: IgG

Application Details

Application Notes:

IHC-IF: 1:50 - 1:1,000

IHC: 1:100 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin/NCL

Background: Background: Nucleolin (NCL) is a major nucleolar phosphoprotein that is involved in pre-RNA transcription and the synthesis and maturation of ribosomes. NCL can bind to histone H1 to induce chromatin condensation and may play an additional role in transcriptional elongation.

Gene ID: 4691

NCBI Accession: NP_005372

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization