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Vinculin antibody (AA 1001-1134) (Cy7)

The Rabbit Polyclonal anti-Vinculin antibody has been validated for WB, FACS, IF (cc) and IF (p). It is suitable to detect Vinculin in samples from Human, Mouse, Rat and Rabbit.
Catalog No. ABIN719267

Quick Overview for Vinculin antibody (AA 1001-1134) (Cy7) (ABIN719267)

Target

See all Vinculin (VCL) Antibodies
Vinculin (VCL)

Reactivity

  • 148
  • 92
  • 70
  • 27
  • 18
  • 5
  • 5
  • 5
  • 5
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
Human, Mouse, Rat, Rabbit

Host

  • 99
  • 53
  • 1
Rabbit

Clonality

  • 86
  • 67
Polyclonal

Conjugate

  • 98
  • 9
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
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  • 2
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  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Vinculin antibody is conjugated to Cy7

Application

  • 115
  • 68
  • 33
  • 30
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  • 22
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  • 19
  • 13
  • 12
  • 9
  • 4
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  • 3
  • 2
  • 1
Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
  • Binding Specificity

    • 26
    • 15
    • 9
    • 7
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
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    • 2
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    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    AA 1001-1134

    Cross-Reactivity

    Human, Mouse, Rabbit, Rat

    Predicted Reactivity

    Dog,Cow,Pig,Horse,Chicken

    Purification

    Purified by Protein A.

    Immunogen

    KLH conjugated synthetic peptide derived from human Vinculin

    Isotype

    IgG
  • Application Notes

    FCM 1:20-100
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 μg/μL

    Buffer

    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    Vinculin (VCL)

    Alternative Name

    Vinculin

    Background

    Synonyms: MV, MVCL, CMD1W, CMH15, HEL114, Vinculin, Metavinculin, VCL

    Background: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

    Gene ID

    7414

    UniProt

    P18206

    Pathways

    Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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