FOXP3 antibody (PE)

Catalog No. ABIN7457173

The Mouse Monoclonal anti-FOXP3 antibody has been validated for ICFC. It is suitable to detect FOXP3 in samples from Mouse.

Quick Overview for FOXP3 antibody (PE) (ABIN7457173)

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This FOXP3 antibody is conjugated to PE

Application: Intracellular Flow Cytometry (ICFC)

Clone: 3G3

Product Details anti-FOXP3 Antibody (PE)

Isotype: IgG1, kappa

Application Details

Application Notes: The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

Comment:

Matching Isotype Control: PE Mouse IgG1, κ Isotype Control (ABIN7469761)

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.05 % Proclin300, 1 % BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.

Target Details for FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Alternative Name: Foxp3

Background: Forkhead box protein P3,IPEX,JM2,Foxp3,FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in 'scurfy' mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence, the other (FOXP3 δ2) lacking exon 2.

Gene ID: 50943, 20371, 317382

UniProt: Q99JB6

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation