HMGB2 antibody (C-Term)

Catalog No. ABIN7464134

This anti-HMGB2 antibody is a Rabbit Polyclonal antibody detecting HMGB2 in WB, IF, IHC (p) and ICC. Suitable for Human.

Quick Overview for HMGB2 antibody (C-Term) (ABIN7464134)

Target: HMGB2 (High Mobility Group Box 2 (HMGB2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-HMGB2 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HMGB2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2 , HMGB2-transfected 293T

Validation: KO/KD, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for HMGB2

Target: HMGB2 (High Mobility Group Box 2 (HMGB2))

Alternative Name: high mobility group box 2

Background: High mobility group box 2 , HMG2,This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. [provided by RefSeq]

Molecular Weight: 24 kDa

Gene ID: 3148

UniProt: P26583

Pathways: Cellular Response to Molecule of Bacterial Origin