DGCR8 antibody (N-Term)

Catalog No. ABIN7464181

The Rabbit Polyclonal anti-DGCR8 antibody has been validated for WB and IP. It is suitable to detect DGCR8 in samples from Human.

Quick Overview for DGCR8 antibody (N-Term) (ABIN7464181)

Target: DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DGCR8 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Grade: KO Validated

Product Details anti-DGCR8 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Frog, Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human DGCR8. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.15 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for DGCR8

Target: DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))

Alternative Name: DGCR8 microprocessor complex subunit

Background: DGCR8 microprocessor complex subunit , C22orf12 , DGCRK6 , Gy1 , pasha,This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

Molecular Weight: 86 kDa

Gene ID: 54487

UniProt: Q8WYQ5

Pathways: Regulatory RNA Pathways