DGCR8 antibody (N-Term)

Catalog No. ABIN2776557

Cited in 3+ publications. The Rabbit Polyclonal anti-DGCR8 antibody (ABIN2776557) specifically detects DGCR8 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Horse, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig and Rabbit samples.

Quick Overview for DGCR8 antibody (N-Term) (ABIN2776557)

Target: DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))

Reactivity: Human, Mouse, Rat, Horse, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DGCR8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-DGCR8 Antibody

Binding Specificity: N-Term

Purpose: DGCR8 Antibody - N-terminal region

Sequence: DKKDEENELD QEKRVEYAVL DELEDFTDNL ELDEEGAGGF TAKAIVQRDR

Predicted Reactivity: Cow: 85%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against DGCR8. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DGCR8

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP40984-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-DGCR8 antibody (ABIN2776557)

Belair, Paikari, Moltzahn, Shenoy, Yau, DallEra, Simko, Benz, Blelloch: "DGCR8 is essential for tumor progression following PTEN loss in the prostate." in: EMBO reports, Vol. 16, Issue 9, pp. 1219-32, (2015) (PubMed).

Van Duyne, Guendel, Klase, Narayanan, Coley, Jaworski, Roman, Popratiloff, Mahieux, Kehn-Hall, Kashanchi: "Localization and sub-cellular shuttling of HTLV-1 tax with the miRNA machinery." in: PLoS ONE, Vol. 7, Issue 7, pp. e40662, (2012) (PubMed).

Kye, Neveu, Lee, Zhou, Steen, Sahin, Kosik, Silva: "NMDA mediated contextual conditioning changes miRNA expression." in: PLoS ONE, Vol. 6, Issue 9, pp. e24682, (2011) (PubMed).

Target Details for DGCR8

Target: DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))

Alternative Name: DGCR8

Background:

Targeted Subunit: DGCR8

Background Information: DGCR8 contains 2 DRBM (double-stranded RNA-binding) domains and 1 WW domain. It may play a part in the etiology of the velocardiofacial/DiGeorge syndrome (VCFS/DGS), a developmental disorder characterized by structural and functional palate anomalies, conotruncal cardiac malformations, immunodeficiency, hypocalcemia, and typical facial anomalies.

Gene Name: DiGeorge syndrome critical region gene 8

Alternative Symbols: Gy1, pasha, DGCRK6, C22orf12

Protein Name: Microprocessor complex subunit DGCR8

Molecular Weight: 86 kDa

Gene ID: 54487

NCBI Accession: NP_073557

UniProt: Q8WYQ5

Pathways: Regulatory RNA Pathways