LIG3 antibody

Catalog No. ABIN7464202

The Mouse Monoclonal anti-LIG3 antibody has been validated for WB, IF, ICC, IP, BP and PLA. It is suitable to detect LIG3 in samples from Human.

Quick Overview for LIG3 antibody (ABIN7464202)

Target: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LIG3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Blocking Peptide (BP), Proximity Ligation Assay (PLA)

Clone: 1F3

Product Details anti-LIG3 Antibody

Cross-Reactivity: Chicken, Human, Mouse

Purification: Protein G affinity purified.

Immunogen: Recombinant full-length human DNA Ligase III-b.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for LIG3

Target: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Alternative Name: DNA ligase 3

Background: DNA ligase 3 , LIG2 , LIG3alpha,This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Molecular Weight: 113 kDa

Gene ID: 3980

UniProt: P49916

Pathways: DNA Damage Repair