GTF2F1 antibody

Catalog No. ABIN7464208

The Rabbit Polyclonal anti-GTF2F1 antibody has been validated for WB, IP and ChIP. It is suitable to detect GTF2F1 in samples from Human.

Quick Overview for GTF2F1 antibody (ABIN7464208)

Target: GTF2F1 (General Transcription Factor IIF, Polypeptide 1, 74kDa (GTF2F1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2F1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Grade: KO Validated

Product Details anti-GTF2F1 Antibody

Specificity: Knockdown/Knockout validation was supported by references (PMID:26990993)

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human TFIIF. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , NIH-3T3

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for GTF2F1

Target: GTF2F1 (General Transcription Factor IIF, Polypeptide 1, 74kDa (GTF2F1))

Alternative Name: general transcription factor IIF subunit 1

Background: General transcription factor IIF subunit 1 , BTF4 , RAP74 , TF2F1 , TFIIF,TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.

Molecular Weight: 58 kDa

Gene ID: 2962

UniProt: P35269