TET1 antibody

Catalog No. ABIN7464226

The Mouse Monoclonal anti-TET1 antibody has been validated for WB, IF, IHC (p) and ICC. It is suitable to detect TET1 in samples from Human.

Quick Overview for TET1 antibody (ABIN7464226)

Target: TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TET1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Grade: KO Validated

Clone: GT1462

Product Details anti-TET1 Antibody

Cross-Reactivity: Human, Mouse

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary.

Isotype: IgG2b

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , DDDDK-human TET1-transfected 293T cells , NT2D1 nuclear extract

Validation: KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for TET1

Target: TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))

Alternative Name: tet methylcytosine dioxygenase 1

Background: Tet methylcytosine dioxygenase 1 , CXXC6 , LCX , bA119F7.1,Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

Molecular Weight: 235 kDa

Gene ID: 80312

UniProt: Q8NFU7

Pathways: Stem Cell Maintenance, Warburg Effect