GLUL antibody

Catalog No. ABIN7464227

This anti-GLUL antibody is a Rabbit Polyclonal antibody detecting GLUL in WB, IHC, IF, IHC (p), ICC, IP, IHC (fro) and IHC (wm). Suitable for Human.

Quick Overview for GLUL antibody (ABIN7464227)

Target: GLUL (Glutamate-Ammonia Ligase (GLUL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLUL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Whole Mount) (IHC (wm))

Grade: KO Validated

Product Details anti-GLUL Antibody

Cross-Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Purification: Purified by antigen-affinity chromatography.

Immunogen: Full length human Glutamine synthetase Recombinant protein.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , DDDDK-tagged GLUL-transfected 293T

Validation: KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for GLUL

Target: GLUL (Glutamate-Ammonia Ligase (GLUL))

Alternative Name: glutamate-ammonia ligase

Background: Glutamate-ammonia ligase , GLNS , GS , PIG43 , PIG59,Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 6.3.1.2), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM]

Molecular Weight: 42 kDa

Gene ID: 2752

UniProt: P15104

Pathways: Positive Regulation of Peptide Hormone Secretion