SHMT2 antibody (N-Term)

Catalog No. ABIN7464230

This anti-SHMT2 antibody is a Rabbit Polyclonal antibody detecting SHMT2 in WB, IF, ICC, IHC (p) and IHC (fro). Suitable for Human.

Quick Overview for SHMT2 antibody (N-Term) (ABIN7464230)

Target: SHMT2 (serine Hydroxymethyltransferase 2 (Mitochondrial) (SHMT2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHMT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SHMT2 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human SHMT2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , HeLa , A431 , HepG2

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SHMT2

Target: SHMT2 (serine Hydroxymethyltransferase 2 (Mitochondrial) (SHMT2))

Alternative Name: serine hydroxymethyltransferase 2

Background: Serine hydroxymethyltransferase 2 , GLYA , HEL-S-51e , SHMT,This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Molecular Weight: 56 kDa

Gene ID: 6472

UniProt: P34897