Topoisomerase I antibody (N-Term)

Catalog No. ABIN7464249

This Rabbit Polyclonal antibody specifically detects Topoisomerase I in WB. It exhibits reactivity toward Human.

Quick Overview for Topoisomerase I antibody (N-Term) (ABIN7464249)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase I antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Topoisomerase I Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Topoisomerase I. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , rat testis

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: DNA topoisomerase I

Background: DNA topoisomerase I , TOPI,This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008]

Molecular Weight: 91 kDa

Gene ID: 7150

UniProt: P11387

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance