beta Actin antibody (C-Term)

Catalog No. ABIN7464288

This Mouse Monoclonal antibody specifically detects beta Actin in WB, IF, IHC (p), ICC and IP. It exhibits reactivity toward Human.

Quick Overview for beta Actin antibody (C-Term) (ABIN7464288)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Grade: KO Validated

Clone: GT5512

Product Details anti-beta Actin Antibody

Binding Specificity: C-Term

Specificity: IP/MS validation was supported by references (PMID:30377426)

Cross-Reactivity: Cat, Dog, Drosophila melanogaster, Goat, Human, Monkey, Mouse, Rabbit, Rat, Saccharomyces cerevisiae, Xenopus laevis, Zebrafish (Danio rerio)

Purification: Affinity purified by Protein G.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 30 hpf zebrafish , Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , NIH-3T3 , mouse brain , PC-12 , rat brain , rabbit blood , goat blood , MDA-MB-231

Validation: IP/MS, KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: actin beta

Background: Actin beta , BRWS1 , PS1TP5BP1,This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Molecular Weight: 42 kDa

Gene ID: 60

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction