ENO1 antibody

Catalog No. ABIN7464349

The Mouse Monoclonal anti-ENO1 antibody has been validated for WB. It is suitable to detect ENO1 in samples from Human.

Quick Overview for ENO1 antibody (ABIN7464349)

Target: ENO1 (Enolase 1 (ENO1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ENO1 antibody is un-conjugated

Application: Western Blotting (WB)

Grade: KO Validated

Clone: GT2217

Product Details anti-ENO1 Antibody

Cross-Reactivity: Human, Rat

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ENO1. The exact sequence is proprietary.

Isotype: IgG2b

Application Details

Application Notes: WB: 1:5000-1:20000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A549 , H1299 , HCT116 , 293T

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ENO1

Target: ENO1 (Enolase 1 (ENO1))

Alternative Name: enolase 1

Background: Enolase 1 , ENO1L1 , HEL-S-17 , MPB1 , NNE , PPH,This gene encodes one of three enolase isoenzymes found in mammals, it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq]

Molecular Weight: 47 kDa

Gene ID: 2023

UniProt: P06733