HIST1H1E antibody (pSer35)

Catalog No. ABIN7464375

This Rabbit Polyclonal antibody specifically detects HIST1H1E in WB, IF, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for HIST1H1E antibody (pSer35) (ABIN7464375)

Target: HIST1H1E (Histone H1.4 (HIST1H1E))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIST1H1E antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HIST1H1E Antibody

Binding Specificity: pSer35

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Ser35 of human Histone H1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Acid-extracted histones from HeLa cell treated Nocodazole

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.93 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for HIST1H1E

Target: HIST1H1E (Histone H1.4 (HIST1H1E))

Alternative Name: histone cluster 1, H1e

Background: H1 histone family antibody , Histone H1.4 phospho Ser36 antibody , Phospho Histone H1.4 antibody , Phospho Histone H1.4 (pSer36) antibody , histone H1b antibody , H1E antibody , H1.4S35ph antibody , "histone 1 antibody , Histone H1.4 (phospho Ser36) antibody , member 4 antibody" antibody , "histone cluster 1 antibody , Histone H1.4 phospho S36 antibody , Histone H1.4 (pS36) antibody , Phospho Histone H1.4 (Ser36) antibody , H1.4 antibody , H1F4 antibody , dJ221C16.5 antibody , MGC116819 antibody , Histone H1.4 (phospho S36) antibody , H1e antibody" antibody , Histone H1.4 (pSer36) antibody , Phospho Histone H1.4 (pS36) antibody , histone H1.4 antibody , HIST1H1E antibody,Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]

Molecular Weight: 22 kDa

Gene ID: 3008