PARP1 antibody (Cleaved-Asp214)

Catalog No. ABIN7464431

This anti-PARP1 antibody is a Rabbit Polyclonal antibody detecting PARP1 in WB, IF and ICC. Suitable for Human.

Quick Overview for PARP1 antibody (Cleaved-Asp214) (ABIN7464431)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-PARP1 Antibody

Binding Specificity: Cleaved-Asp214

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human PARP (cleaved Asp214). The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HCT116 (30uM cisplatin treatment for 24hr) , 293T

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.33 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: poly(ADP-ribose) polymerase 1

Background: Poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1,This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

Molecular Weight: 113 kDa

Gene ID: 142

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location