TUBB3 antibody (C-Term)

Catalog No. ABIN7464472

The Mouse Monoclonal anti-TUBB3 antibody has been validated for WB, IF, IHC (p), IP, ICC and IHC (fro). It is suitable to detect TUBB3 in samples from Human.

Quick Overview for TUBB3 antibody (C-Term) (ABIN7464472)

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Grade: KO Validated

Clone: GT11710

Product Details anti-TUBB3 Antibody

Binding Specificity: C-Term

Specificity: Beta III Tubulin antibody [GT11710] specifically recognizes endogenous levels of beta III Tubulin based on the immunogen sequence.

Cross-Reactivity: Fish, Human, Mouse, Rat

Purification: Affinity purified by Protein A.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Tubulin 3/ Tuj1. The exact sequence is proprietary.

Isotype: IgG2b

Application Details

Application Notes: WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for TUBB3

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Alternative Name: tubulin beta 3 class III

Background: Tubulin beta 3 class III , CDCBM , CDCBM1 , CFEOM3 , CFEOM3A , FEOM3 , TUBB4 , beta-4,This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq]

Molecular Weight: 50 kDa

Gene ID: 10381

UniProt: Q13509

Pathways: Microtubule Dynamics, M Phase