PGK1 antibody

Catalog No. ABIN7464481

The Rabbit Polyclonal anti-PGK1 antibody has been validated for WB, ELISA, IF, IHC (p), ICC, IP and PLA. It is suitable to detect PGK1 in samples from Human.

Quick Overview for PGK1 antibody (ABIN7464481)

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PGK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP), Proximity Ligation Assay (PLA)

Grade: KO Validated

Product Details anti-PGK1 Antibody

Cross-Reactivity: Human, Mouse, Rat, Saccharomyces cerevisiae

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:20-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa (1% O2 treatment for 24hr) , Mouse brain , Rat liver , 293T , A431 , HeLa , HepG2 , PGK1-transfected 293T

Validation: KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.048 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PGK1

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Alternative Name: phosphoglycerate kinase 1

Background: Phosphoglycerate kinase 1 , HEL-S-68p , MIG10 , PGKA,The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]

Molecular Weight: 45 kDa

Gene ID: 5230

UniProt: P00558

Pathways: Cellular Glucan Metabolic Process