HIST1H3A antibody (pSer10)

Catalog No. ABIN7464484

The Mouse Monoclonal anti-HIST1H3A antibody has been validated for WB, IF, ICC and IHC (p). It is suitable to detect HIST1H3A in samples from Human.

Quick Overview for HIST1H3A antibody (pSer10) (ABIN7464484)

Target: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HIST1H3A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: GT921

Product Details anti-HIST1H3A Antibody

Binding Specificity: pSer10

Cross-Reactivity: Human, Mouse, Rat

Purification: Affinity purified by Protein G.

Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Ser10 of human Histone H3. The exact sequence is proprietary.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa (100 ng/ml Nocodazole treatment for 24 hr)

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for HIST1H3A

Target: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Alternative Name: histone cluster 1, H3a

Background: Histone cluster 1 , H3a Antibody , H3/A Antibody , HIST1H3A Antibody , H3FA Antibody,Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]

Gene ID: 8350