CA9 antibody (N-Term)

Catalog No. ABIN7464487

The Mouse Monoclonal anti-CA9 antibody has been validated for WB, IF and ICC. It is suitable to detect CA9 in samples from Human.

Quick Overview for CA9 antibody (N-Term) (ABIN7464487)

Target: CA9 (Carbonic Anhydrase IX (CA9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CA9 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: GT14212

Product Details anti-CA9 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Carbonic Anhydrase IX. The exact sequence is proprietary.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa (200 μM CoCl2 treatment for 24 hr) , 30u g HeLa (200 μM CoCl2 treatment for 48 hr) , HeLa (1% O2 treatment for 24 hr) , HeLa (1% O2 treatment for 48 hr)

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for CA9

Target: CA9 (Carbonic Anhydrase IX (CA9))

Alternative Name: carbonic anhydrase 9

Background: Carbonic anhydrase 9 , CAIX , MN,Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq]

Molecular Weight: 50 kDa

Gene ID: 768

UniProt: Q16790