GTF2B antibody

Catalog No. ABIN7464504

This Rabbit Polyclonal antibody specifically detects GTF2B in WB, IF, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for GTF2B antibody (ABIN7464504)

Target: GTF2B (General Transcription Factor IIB (GTF2B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-GTF2B Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human TFIIB. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa nuclear extract , GTF2B-transfected 293T , 293T , A431 , HeLa , HepG2

Validation: Comparison, Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.56 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for GTF2B

Target: GTF2B (General Transcription Factor IIB (GTF2B))

Alternative Name: general transcription factor IIB

Background: General transcription factor IIB , TF2B , TFIIB,This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq]

Molecular Weight: 35 kDa

Gene ID: 2959

UniProt: Q00403