WIPI1 antibody (C-Term)

Catalog No. ABIN7464565

The Rabbit Polyclonal anti-WIPI1 antibody has been validated for WB. It is suitable to detect WIPI1 in samples from Human.

Quick Overview for WIPI1 antibody (C-Term) (ABIN7464565)

Target: WIPI1 (WD Repeat Domain, phosphoinositide Interacting 1 (WIPI1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WIPI1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-WIPI1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human WIPI1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:20000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HepG2 , human WIPI1-transfected 293T cells

Validation: Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for WIPI1

Target: WIPI1 (WD Repeat Domain, phosphoinositide Interacting 1 (WIPI1))

Alternative Name: WD repeat domain, phosphoinositide interacting 1

Background: WD repeat domain, phosphoinositide interacting 1 , ATG18 , ATG18A , WIPI49,WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI1, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM]

Molecular Weight: 49 kDa

Gene ID: 55062

UniProt: Q5MNZ9

Pathways: Nuclear Hormone Receptor Binding, ER-Nucleus Signaling