EPAS1 antibody

Catalog No. ABIN7464570

This Mouse Monoclonal antibody specifically detects EPAS1 in WB, IP, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for EPAS1 antibody (ABIN7464570)

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPAS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: GT125

Product Details anti-EPAS1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the center region of human HIF2 alpha. The exact sequence is proprietary.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:700. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HepG2 (1% O2 treatment for 24hr)

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, No preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Alternative Name: endothelial PAS domain protein 1

Background: Endothelial PAS domain protein 1 , ECYT4 , HIF2A , HLF , MOP2 , PASD2 , bHLHe73,This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]

Molecular Weight: 96 kDa

Gene ID: 2034

UniProt: Q99814

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect