ARHGEF2 antibody (C-Term)

Catalog No. ABIN7464598

The Rabbit Polyclonal anti-ARHGEF2 antibody is suitable to detect ARHGEF2 in samples from Human. It has been validated for WB, IF, IHC (p), ICC, IP and IHC (fro).

Quick Overview for ARHGEF2 antibody (C-Term) (ABIN7464598)

Target: ARHGEF2 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 2 (ARHGEF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Grade: KO Validated

Product Details anti-ARHGEF2 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human GEF-H1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: hMSC-3A6 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , 293T , A431 , HeLa , HepG2 , PC-12 , Rat2

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.51 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ARHGEF2

Target: ARHGEF2 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 2 (ARHGEF2))

Alternative Name: Rho/Rac guanine nucleotide exchange factor 2

Background: Rho/Rac guanine nucleotide exchange factor 2 , GEF , GEF-H1 , GEFH1 , LFP40 , NEDMHM , P40,Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. [provided by RefSeq]

Molecular Weight: 112 kDa

Gene ID: 9181

UniProt: Q92974

Pathways: Negative Regulation of intrinsic apoptotic Signaling