Rhotekin antibody (N-Term)

Catalog No. ABIN7464752

This anti-Rhotekin antibody is a Rabbit Polyclonal antibody detecting Rhotekin in WB, IF and ICC. Suitable for Human.

Quick Overview for Rhotekin antibody (N-Term) (ABIN7464752)

Target: Rhotekin (RTKN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Rhotekin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Rhotekin Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Rhotekin. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , Jurkat , Raji , NCI-H929 , human Rhotekin-transfected 293T cells

Validation: Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.54 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Rhotekin

Target: Rhotekin (RTKN)

Alternative Name: rhotekin

Background: Rhotekin,This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Molecular Weight: 63 kDa

Gene ID: 6242

UniProt: Q9BST9