ESR2 antibody (AA 1-153)

Catalog No. ABIN7464811

The Mouse Monoclonal anti-ESR2 antibody has been validated for WB, IHC, IF, FACS, ICC, IHC (p), IHC (fro), ChIP, IHC (fp) and DB. It is suitable to detect ESR2 in samples from Human.

Quick Overview for ESR2 antibody (AA 1-153) (ABIN7464811)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ESR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Dot Blot (DB)

Grade: KO Validated

Clone: 14C8

Product Details anti-ESR2 Antibody

Binding Specificity: AA 1-153

Specificity: Strong Erb staining in epithelial cell nuclei. Occasional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also successfully detected ERb in colon and ovarian tumors using the same antibody.,Knockdown/Knockout validation was supported by references (PMID:27323858)

Cross-Reactivity: Human, Monkey, Mouse

Purification: Protein G purified

Immunogen: Amino acids 1-153 of human ER-beta expressed in E. coli.

Isotype: IgG2b

Application Details

Application Notes: For IHC, a recommended protocol is provided. For WB: Use at a concentration of 1-5 μg/mL. For ChIP: Use at an dependent assay. Optimal dilutions/concentrations should be determined by the researcher.

Comment:

Positive Control: Myc-DDK-tagged ESR2-transfected 293T

Validation: Comparison, KO/KD, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.23 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Alternative Name: estrogen receptor 2

Background: Estrogen receptor 2 , ER-BETA , ESR-BETA , ESRB , ESTRB , Erb , NR3A2 , ODG8,This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]

Molecular Weight: 59 kDa

Gene ID: 2100

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling