NFYB antibody

Catalog No. ABIN7464824

The Rabbit Polyclonal anti-NFYB antibody (ABIN7464824) specifically detects NFYB in WB, IF, IP, IHC (p), ICC, ChIP and IHC (wm). The antibody is reactive with Human samples.

Quick Overview for NFYB antibody (ABIN7464824)

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Whole Mount) (IHC (wm))

Product Details anti-NFYB Antibody

Cross-Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NFYB. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.42 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NFYB

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Alternative Name: nuclear transcription factor Y subunit beta

Background: Nuclear transcription factor Y subunit beta , CBF-A , CBF-B , HAP3 , NF-YB,The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence, protein sequence alignments and experiments with mutants. [provided by RefSeq]

Molecular Weight: 23 kDa

Gene ID: 4801

UniProt: P25208

Pathways: Regulation of Lipid Metabolism by PPARalpha