PPP4C antibody (Catalytic Subunit)

Catalog No. ABIN7464842

This anti-PPP4C antibody is a Rabbit Polyclonal antibody detecting PPP4C in WB, IF and ICC. Suitable for Human.

Quick Overview for PPP4C antibody (Catalytic Subunit) (ABIN7464842)

Target: PPP4C (Protein Phosphatase 4, Catalytic Subunit (PPP4C))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP4C antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-PPP4C Antibody

Binding Specificity: Catalytic Subunit

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PPP4C. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3 , JC , BCL-1 , HepG2

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.29 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PPP4C

Target: PPP4C (Protein Phosphatase 4, Catalytic Subunit (PPP4C))

Alternative Name: protein phosphatase 4

Background: Protein phosphatase 4 catalytic subunit , PP-X , PP4 , PP4C , PPH3 , PPP4 , PPX,Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase.

Molecular Weight: 35 kDa

Gene ID: 5531

UniProt: P60510