EIF4B antibody (N-Term)

Catalog No. ABIN7464855

This Rabbit Polyclonal antibody specifically detects EIF4B in WB, IF, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for EIF4B antibody (N-Term) (ABIN7464855)

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-EIF4B Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human eIF4B. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NT2D1 , IMR32 , U87-MG , MCF-7 , U87-MG , SK-N-SH , IMR32 , SK-N-AS , human EIF4B-transfected 293T , 293T , A431 , HeLa , HepG2

Validation: Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.16 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for EIF4B

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Alternative Name: eukaryotic translation initiation factor 4B

Background: Eukaryotic translation initiation factor 4B , EIF-4B , PRO1843,Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Molecular Weight: 69 kDa

Gene ID: 1975

UniProt: P23588