FH antibody

Catalog No. ABIN7464916

The Mouse Monoclonal anti-FH antibody has been validated for WB, IF and ICC. It is suitable to detect FH in samples from Human.

Quick Overview for FH antibody (ABIN7464916)

Target: FH (Fumarate Hydratase (FH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: KO Validated

Clone: GT7612

Product Details anti-FH Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Fumarate hydratase. The exact sequence is proprietary.

Isotype: IgG3

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: rat liver , mouse liver , 293T , A431 , HeLa , HepG2

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for FH

Target: FH (Fumarate Hydratase (FH))

Alternative Name: fumarate hydratase

Background: Fumarate hydratase , FMRD , HLRCC , LRCC , MCL , MCUL1,The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008]

Molecular Weight: 55 kDa

Gene ID: 2271

UniProt: P07954