ISG15 antibody

Catalog No. ABIN7464925

The Rabbit Polyclonal anti-ISG15 antibody is suitable to detect ISG15 in samples from Human. It has been validated for WB, IF, ICC and IHC (p).

Quick Overview for ISG15 antibody (ABIN7464925)

Target: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ISG15 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Product Details anti-ISG15 Antibody

Cross-Reactivity: Human, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ISG15. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: rat spleen , HeLa

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.45 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ISG15

Target: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Alternative Name: ISG15 ubiquitin like modifier

Background: ISG15 ubiquitin like modifier , G1P2 , IFI15 , IMD38 , IP17 , UCRP , hUCRP,ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA, MIM 147660) and -beta (IFNB, MIM 147640) (Zhao et al., 2005 [PubMed 16009940]).[supplied by OMIM]

Molecular Weight: 18 kDa

Gene ID: 9636

UniProt: P05161