Lamin B2 antibody

Catalog No. ABIN7464965

This anti-Lamin B2 antibody is a Mouse Monoclonal antibody detecting Lamin B2 in WB, IF, IHC (p), IP and ICC. Suitable for Human.

Quick Overview for Lamin B2 antibody (ABIN7464965)

Target: Lamin B2 (LMNB2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lamin B2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Grade: KO Validated

Clone: GT144

Product Details anti-Lamin B2 Antibody

Cross-Reactivity: Human

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Lamin B2. The exact sequence is proprietary.

Isotype: IgG2a

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: U87-MG , SK-N-SH , IMR32 , SK-N-AS , HepG2 , HepG2 nuclear

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.81 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Lamin B2

Target: Lamin B2 (LMNB2)

Alternative Name: lamin B2

Background: Lamin B2 , EPM9 , LAMB2 , LMN2,The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B2. This gene is in a head-to-tail orientation with the gene for the translocase of inner mitochondrial membrane 13 homolog gene. [provided by RefSeq]

Molecular Weight: 68 kDa

Gene ID: 84823

UniProt: Q03252

Pathways: Apoptosis, Caspase Cascade in Apoptosis