TUBB3 antibody

Catalog No. ABIN7464966

This Rabbit Polyclonal antibody specifically detects TUBB3 in WB, FACS, IF, IHC (p), IP, ICC and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for TUBB3 antibody (ABIN7464966)

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUBB3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Grade: KO Validated

Product Details anti-TUBB3 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human beta Tubulin 3/ TUJ1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:50000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain , HeLa

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for TUBB3

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Alternative Name: tubulin beta 3 class III

Background: Tubulin beta 3 class III , CDCBM , CDCBM1 , CFEOM3 , CFEOM3A , FEOM3 , TUBB4 , beta-4,This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq]

Molecular Weight: 50 kDa

Gene ID: 10381

UniProt: Q13509

Pathways: Microtubule Dynamics, M Phase