TBP antibody (N-Term)

Catalog No. ABIN7465077

This Mouse Monoclonal antibody specifically detects TBP in WB. It exhibits reactivity toward Human.

Quick Overview for TBP antibody (N-Term) (ABIN7465077)

Target: TBP (TATA Box Binding Protein (TBP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TBP antibody is un-conjugated

Application: Western Blotting (WB)

Clone: GT453

Product Details anti-TBP Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human

Purification: Affinity purified by Protein G.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human TBP. The exact sequence is proprietary.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for TBP

Target: TBP (TATA Box Binding Protein (TBP))

Alternative Name: TATA-box binding protein

Background: TATA-box binding protein , GTF2D , GTF2D1 , HDL4 , SCA17 , TFIID,Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. [provided by RefSeq]

Molecular Weight: 38 kDa

Gene ID: 6908

UniProt: P20226

Pathways: WNT Signaling