DLAT antibody

Catalog No. ABIN7465116

This Rabbit Polyclonal antibody specifically detects DLAT in WB, IF, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for DLAT antibody (ABIN7465116)

Target: DLAT (Dihydrolipoyl Transacetylase (DLAT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLAT antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DLAT Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human DLAT. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for DLAT

Target: DLAT (Dihydrolipoyl Transacetylase (DLAT))

Alternative Name: dihydrolipoamide S-acetyltransferase

Background: Dihydrolipoamide S-acetyltransferase , DLTA , E2 , PDC-E2 , PDCE2,The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC, EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC, MIM 109720) show autoantibodies to DLAT.[supplied by OMIM]

Molecular Weight: 69 kDa

Gene ID: 1737

UniProt: P10515