USP20 antibody

Catalog No. ABIN7465301

This anti-USP20 antibody is a Rabbit Polyclonal antibody detecting USP20 in WB. Suitable for Human.

Quick Overview for USP20 antibody (ABIN7465301)

Target: USP20 (Ubiquitin Specific Peptidase 20 (USP20))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP20 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-USP20 Antibody

Cross-Reactivity: Human

Purification: Affinity purified by Protein A.

Immunogen: Recombinant protein encompassing a sequence within the center region of human USP20. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.98 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for USP20

Target: USP20 (Ubiquitin Specific Peptidase 20 (USP20))

Alternative Name: ubiquitin specific peptidase 20

Background: Ubiquitin specific peptidase 20 , LSFR3A , VDU2 , hVDU2,Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Molecular Weight: 102 kDa

Gene ID: 10868

UniProt: Q9Y2K6

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling