MRPS18B antibody

Catalog No. ABIN7465369

The Rabbit Polyclonal anti-MRPS18B antibody has been validated for WB and IHC (p). It is suitable to detect MRPS18B in samples from Human.

Quick Overview for MRPS18B antibody (ABIN7465369)

Target: MRPS18B (Mitochondrial Ribosomal Protein S18B (MRPS18B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPS18B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MRPS18B Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human MRPS18B. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.84 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MRPS18B

Target: MRPS18B (Mitochondrial Ribosomal Protein S18B (MRPS18B))

Alternative Name: mitochondrial ribosomal protein S18B

Background: Mitochondrial ribosomal protein S18B , C6orf14 , HSPC183 , HumanS18a , MRP-S18-2 , MRPS18-2 , PTD017 , S18amt,Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The encoded protein is one of three that has significant sequence similarity to bacterial S18 proteins. The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq]

Molecular Weight: 29 kDa

Gene ID: 28973

UniProt: Q9Y676