MED16 antibody

Catalog No. ABIN7465442

This anti-MED16 antibody is a Rabbit Polyclonal antibody detecting MED16 in WB. Suitable for Human.

Quick Overview for MED16 antibody (ABIN7465442)

Target: MED16 (Mediator Complex Subunit 16 (MED16))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED16 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MED16 Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human THRAP5. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431 , HeLa , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MED16

Target: MED16 (Mediator Complex Subunit 16 (MED16))

Alternative Name: mediator complex subunit 16

Background: Mediator complex subunit 16 , DRIP92 , THRAP5 , TRAP95,Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Molecular Weight: 97 kDa

Gene ID: 10025

UniProt: Q9Y2X0

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha