NFYA antibody

Catalog No. ABIN7465454

The Rabbit Polyclonal anti-NFYA antibody (ABIN7465454) specifically detects NFYA in WB, IF, ICC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for NFYA antibody (ABIN7465454)

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NFYA Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Full length human NFYA Recombinant protein.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1 , PC-12 , Rat2 , 293T

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.19 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NFYA

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Alternative Name: nuclear transcription factor Y subunit alpha

Background: Nuclear transcription factor Y subunit alpha , CBF-A , CBF-B , HAP2 , NF-YA,The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq]

Molecular Weight: 37 kDa

Gene ID: 4800

UniProt: P23511

Pathways: Regulation of Lipid Metabolism by PPARalpha