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MED18 antibody

This Rabbit Polyclonal antibody specifically detects MED18 in WB, IHC (p), IF and ICC. It exhibits reactivity toward Human.
Catalog No. ABIN7465530

Quick Overview for MED18 antibody (ABIN7465530)

Target

See all MED18 Antibodies
MED18 (Mediator Complex Subunit 18 (MED18))

Reactivity

  • 55
  • 31
  • 23
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
Human

Host

  • 54
  • 1
Rabbit

Clonality

  • 55
Polyclonal

Conjugate

  • 20
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  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This MED18 antibody is un-conjugated

Application

  • 23
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  • 13
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  • 5
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  • 2
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  • 1
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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)
  • Cross-Reactivity

    Human

    Purification

    Purified by antigen-affinity chromatography.

    Immunogen

    Recombinant protein encompassing a sequence within the center region of human MED18. The exact sequence is proprietary.

    Isotype

    IgG
  • Application Notes

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    Comment

    Positive Control: A549

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.67 mg/mL

    Buffer

    0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

    Preservative

    Thimerosal (Merthiolate)

    Precaution of Use

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Target

    MED18 (Mediator Complex Subunit 18 (MED18))

    Alternative Name

    mediator complex subunit 18

    Background

    Mediator complex subunit 18 , SRB5 , p28b,MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied by OMIM]

    Molecular Weight

    24 kDa

    Gene ID

    54797

    UniProt

    Q9BUE0

    Pathways

    Regulation of Lipid Metabolism by PPARalpha
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