MED27 antibody

Catalog No. ABIN7465565

This anti-MED27 antibody is a Rabbit Polyclonal antibody detecting MED27 in WB. Suitable for Human.

Quick Overview for MED27 antibody (ABIN7465565)

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED27 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MED27 Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human MED27. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A549

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.82 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MED27

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Alternative Name: mediator complex subunit 27

Background: Mediator complex subunit 27 , CRAP34 , CRSP34 , CRSP8 , MED3 , TRAP37,The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq]

Molecular Weight: 35 kDa

Gene ID: 9442

UniProt: Q6P2C8

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha