SAFB antibody (AA 345-357)

Catalog No. ABIN7466004

This Mouse Monoclonal antibody specifically detects SAFB in WB, IHC, IF, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for SAFB antibody (AA 345-357) (ABIN7466004)

Target: SAFB (Scaffold Attachment Factor B (SAFB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SAFB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 6F7

Product Details anti-SAFB Antibody

Binding Specificity: AA 345-357

Cross-Reactivity: Human, Mouse

Purification: Protein G purified

Immunogen: A synthetic peptide corresponding to amino acids 345-357 (PEARDSKEDGRKF) of the human SAFB protein.

Isotype: IgG1

Application Details

Application Notes: WB: 1 - 2 μg/mL. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa, MCF7

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SAFB

Target: SAFB (Scaffold Attachment Factor B (SAFB))

Alternative Name: scaffold attachment factor B

Background: Scaffold attachment factor B , HAP , HET , SAB-B1 , SAF-B , SAF-B1 , SAFB1,This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2011]

Molecular Weight: 103 kDa

Gene ID: 6294

UniProt: Q15424

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway