Coagulation Factor X antibody

Catalog No. ABIN7466218

The Mouse Monoclonal anti-Coagulation Factor X antibody has been validated for WB, ICC and IF. It is suitable to detect Coagulation Factor X in samples from Human.

Quick Overview for Coagulation Factor X antibody (ABIN7466218)

Target: Coagulation Factor X (F10)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Coagulation Factor X antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: GT268

Product Details anti-Coagulation Factor X Antibody

Cross-Reactivity: Human

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Factor X. The exact sequence is proprietary.

Isotype: IgG2b

Application Details

Application Notes: WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: human F10-transfected 293T cells

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Coagulation Factor X

Target: Coagulation Factor X (F10)

Alternative Name: coagulation factor X

Background: Coagulation factor X , FX , FXA,This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds, the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. [provided by RefSeq]

Molecular Weight: 55 kDa

Gene ID: 2159

UniProt: P00742