Caspase 10 antibody

Catalog No. ABIN7466458

The Rabbit Polyclonal anti-Caspase 10 antibody has been validated for WB, IF, ICC and IHC (p). It is suitable to detect Caspase 10 in samples from Human.

Quick Overview for Caspase 10 antibody (ABIN7466458)

Target: Caspase 10 (CASP10) (Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 10 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Caspase 10 Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Caspase 10. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Jurkat , NCI-H929

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Caspase 10

Target: Caspase 10 (CASP10) (Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10))

Alternative Name: caspase 10

Background: Caspase 10 , ALPS2 , FLICE2 , MCH4,This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Molecular Weight: 59 kDa

Gene ID: 843

UniProt: Q92851

Pathways: Apoptosis, Caspase Cascade in Apoptosis