HLCS antibody

Catalog No. ABIN7466527

The Rabbit Polyclonal anti-HLCS antibody has been validated for WB and IHC (p). It is suitable to detect HLCS in samples from Human.

Quick Overview for HLCS antibody (ABIN7466527)

Target: HLCS (Biotin-Protein Ligase (HLCS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLCS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HLCS Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human HCS. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: K562

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for HLCS

Target: HLCS (Biotin-Protein Ligase (HLCS))

Alternative Name: holocarboxylase synthetase

Background: Holocarboxylase synthetase , HCS,Holocarboxylase synthetase (EC 6.3.4.10) covalently links biotin to propionyl-CoA carboxylase (PCCA, MIM 232000), pyruvate carboxylase (PC, MIM 608786), alpha-methylcrotonyl-CoA carboxylase (MCCC1, MIM 609010), and acetyl-CoA carboxylase (ACACA, MIM 200350).[supplied by OMIM]

Molecular Weight: 81 kDa

Gene ID: 3141

UniProt: P50747