NCK1 antibody

Catalog No. ABIN7466528

The Rabbit Polyclonal anti-NCK1 antibody (ABIN7466528) specifically detects NCK1 in WB, IF, ICC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for NCK1 antibody (ABIN7466528)

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NCK1 Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NCK1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: K562

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.78 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NCK1

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Alternative Name: NCK adaptor protein 1

Background: NCK adaptor protein 1 , NCK , NCKalpha , nck-1,The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. [provided by RefSeq]

Molecular Weight: 43 kDa

Gene ID: 4690

UniProt: P16333

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals